Comprehensive Standardization And Assessment of Behavioural Core In Rodants Of Ethanolic Extract Of Floral Part of Delonix regia (bojer ex hook.) Raf. Of Pakistan

Raheela Khursheed, Ghazala. H. Rizwani, Hina Zahid , Sumaira Ishaque

—  Delonix regia is branched, broad, spreading, flat- crowned deciduous tree and it is well known for its brilliant display of red- orange bloom in the form of bunch. The extract of different parts of plant has been used in the treatment of malaria, bacterial infections and constipation. The present investigation was conducted to standardize the drug for quality assessment and in addition to evaluate its behavioural core in rodent. In standardization of plant material the pharmacognostic techniques (Macroscopic, microscopic, histological characteristics, Physico chemical parameters, ash values along with extractive values) was carried out. Macroscopic studies indicated the presence of five thick crimson sepals curve back to display their lime- green lining and five spoons shaped petals, one of them is larger having streaked centre.  Microscopically, the transverse section of Delonix regia flower showed the presence of single layer of barrel shaped cells with stomata. Cells containing colouring pigments were also present. Powdered flowers material revealed the presence of Oil cells, Fibres, Fragments of vascular tissues, ca-oxalate crystals, starch granules, cork tissues epidermal and stone cells. Physico chemical parameters including total ash value which was 6.51% w/w,  acid insoluble ash was not more than 8.4% w/w, water soluble ash is equivalent to 7.44% w/w and sulphated ash is 16.76 % w/w. Alcohol soluble extractive value was more than water soluble extractive value. Phytochemical analysis showed presence of steroids, alkaloids, flavonoids, proteins, tannins, carbohydrates, phenol and triterpenes. Infrared spectroscopic analysis revealed the presence of O-H, C-H, N-H, C=O, C-N and C-O. In case of behavioural study, rotarod showed considerable  lack in motor coordination at 100 mg / kg on 30 and 60^th mins of duration which was 44% and 32.2% respectively while in case of grip strength all tested doses were found ineffective in related to muscle relaxant property