Novel Peptide Suppresses Gene Expressions of C-MYC and CCND1 in Kasumi-1 Cell Lines

ChiyoMizuochi-Yanagi, Akane Yonehara, Yuka Tanaka, AnthonySwain, Toshiyuki Tsunoda, Senji Shirasawaand, Daisuke Sugiyama

Leukemias are disorders that cause the abnormal growth and differentiation of hematopoietic cells. Therapies for leukemia are often invasive and there is no guarantee of a positive outcome. Biologically active peptides are being used for a number of therapies for different diseases. We derived several biologically active peptides from fetal liver derived Delta like-1+ hepatoblasts with the intention of finding novel biologically active peptides for the treatment of leukemia. After screening 9 peptides we discovered that two of the peptides caused changes of cell number in the human leukemic cell line Kasumi-1. Further investigation showed that a peptide with the sequence RRRRRRRR(PEG3)CQKKDGPCVINGS also caused suppression of the C-MYC and CCND1 genes at 1 day. The cell number and viability at 1 day along with the gene expression data suggests the peptide is a novel biologically active peptide that will be useful for the investigation of novel therapies for leukemia.