Simultaneous Analysis of Moxifloxacin and Its Intermediate (4aS,7aS)-1H-octahydropyrrolo [3,4-b]pyridine by UHPLC Coupled with time-of-flight Mass Spectrometry

Hui Ding, Jie Guo, Hua Wang

Moxifloxacin, intermediate, (4aS,7aS)-1H- octahydropyrrolo[3,4-b]pyridine, UHPLC-MS.

A fast UHPLC-MS method was developed to detect moxifloxacin and its intermediate (4aS,7aS)-1H- octahydropyrrolo [3,4-b]pyridine (intermediate 1) simulta- neoussly for the first time. The moxifloxacin and intermediate 1 were separated within 2.0 min. The reliability of the method was demonstrated with the specificity, precision, and accuracy. Based on the peak area in the extracted ion chromatography (EIC), the linear regressions for moxifloxacin and intermediate 1 were both calibrated. In nine replicated trials, the relative standard deviation values for two analytes were both less than 5%. The average recovery of moxifloxacin at 30 ng/mL was 97.10%, while that of intermediate 1 at 3 ng/mL was 98.28%. The concrete samples demonstrated that this unique method can be applied in the producing process and quality control of moxifloxacin.

https://doi.org/10.31871/IJNTR.8.8.10